Table 2 of Mookherjee, Mol Vis 2011; 17:2618-2627.


Table 2. PCR primers, annealing temperature and RFLP pattern for genotyping of WDR36 SNPs.


SNP ID and location in the gene

Primer sequences
Annealing
temperature
Restriction
enzyme
RFLP
pattern
rs1971050 (c.330+925 C>T) 5′-GAGGTGAAGAGCAATTGGGTTTCTC-3′ 60 °C AluI C: 203 bp, 35 bp
T: 238 bp
  5′-GCAGTGTCAGGAAAGACACTGTACC-3′      
rs1993465 (c.459+183A>G) 5′-TTCTTTACCCAGCACACTCTGGAA-3′ 56 °C HinfI G: 147 bp, 25 bp
A: 172 bp
  5′-TTTTGAAGAAGGTCTCCAAGTGATT-3′      
rs13153937 (c.460–112A>G) 5′-GCAGATGAACATGCCTGGTCCCTTA-3′ 58 °C BciVI G: 397 bp
A: 336 bp, 61 bp
  5′-ACAGGCAAAATCTCTGGCATAG-3′      
rs10038177 (c.710+30C>T) 5′-GCCTCTCATTTATTTTATTTCTCAAGG-3′ 62 °C AluI C: 338 bp, 120 bp
T: 458 bp
  5′-CCTCTGATACAGGGGACCAACTG-3′      
rs11241095
(c 772G>A, I264V) 5′-GAGGATGGGATGTTGACTGGCAGA-3′ 50 °C HpaI G: 268 bp, 23 bp
A: 293 bp
  5′-ATAACTTGACCTGACATAAGGTTAA-3′      
rs10043631 (c.1494+90C>T) 5′-TCCCTAGTGTCTGAAATATTGGATGC-3′ 50 °C DdeI C: 328 bp, 25 bp T: 353 bp
  5′-TAAAATGTTAAACTACGTTTTCTCA-3′      
rs10038058 (c.1494+143G>A) 5′-TCTTGTTATACTCAAGGTTTCTCATCA-3′ 50 °C ApoI G: 191 bp, 63 bp
A: 102 bp, 89 bp, 63 bp
  5′-AGAATAGTCTTTTGAACAAATAGTGCTG-3′      
rs10491424 (c.1965–905C>T) 5′-CTATCTCCTCTTAAGTGGTTAATGAATT-3′ 58 °C EcoRI C: 147 bp, 24 bp
T: 171 bp
  5′-ATGTGGTGGATAAGGCAAGG-3′      
rs17553936 (c.1965–30A>G) 5′-TGGTGACTTTCTGATCAATGCTGGTG-3′ 62 °C CvIQI G: 308 bp, 120 bp
A: 428 bp
  5′-TCAAGCAGATGTTGAATCACTCAGA-3′      
rs13186912 (c.2181A>T; V727V) 5′-GGTGTTTTGGGTCTAGTGATGG-3′ 56 °C BstBI A: 234 bp
T: 210 bp, 24 bp
  5′ -CACTTGGTTCTACTGTTTCTTTCGA-3′