Table 1 of
Yan, Mol Vis 2011; 17:2612-2617. Table 1 of
Yan, Mol Vis 2011; 17:2612-2617.
Table 1. Primers used in polymerase chain reaction for amplification of PAX6.
Exon |
Primer direction |
Sequence (5′→3′) |
Annealing temperature (°C) |
Product size (bp) |
|---|---|---|---|---|
| 4 | Forward: | AAGGGTAGATTTTGTATGCAC | 54 | 276 |
| Reverse: | GAAGTCCCAGAAAGACCAGA | |||
| 5 | Forward: | CCTCTTCACTCTGCTCTCTT | 54 | 257 |
| Reverse: | ATGAAGAGAGGGCGTTGAGA | |||
| 5a and 6 | Forward: | TGAAAGTATCATCATATTTGTAG | 54 | 515 |
| Reverse: | AGGAGAGAGCATTGGGCTTA | |||
| 7 | Forward: | CAGGAGACACTACCATTTGG | 56 | 265 |
| Reverse: | GACAGGCAAAGGGATGCAC | |||
| 8 | Forward: | GGGAATGTTTTGGTGAGGCT | 54 | 346 |
| Reverse: | TCTTTGTACTGAAGATGTGGC | |||
| 9 | Forward: | GTAGTTCTGGCACAATATGG | 51 | 329 |
| Reverse: | GCACTGTGTCTACGTCGAG | |||
| 10 and 11 | Forward: | CTCGACGTAGACACAGTGC | 54 | 437 |
| Reverse: | TTATGCAGGCCACCACCAGC | |||
| 12 | Forward: | GCTGTGTGATGTGTTCCTCA | 54 | 245 |
| Reverse: | AAGAGAGATCGCCTCTGTGC |