Figure 5. Western blot analyses of smelt orthologs of mammalian Tbdn and ZO-1 proteins. A: A Tbdn western blot analysis showing a major band of approximately 103 kDa in smelt and 100 kDa in primate cells reacting with affinity purified rabbit anti-mouse Tbdn C10–20 antibody, which are completely competed out by the addition of the C10–20 Tbdn peptide, but not by a control peptide, as indicated. The bottom panel shows Tubulin as a protein loading and integrity control. B: A ZO-1 western blot analysis showing a doublet in primate and smelt cells reacting with the anti-ZO-1 antibody, as indicated. The bottom panel shows Tubulin as a protein loading and integrity control.