Figure 1. Gene expression of ciliary epithelium (CE)-derived cells determined by RT–PCR. RNA was isolated from passage 1 and was subjected to conventional RT–PCR. PCR products were resolved on 1.5% agarose gel. A: Amplification of mRNA for pluripotency markers. B: Amplification of mRNA for retinal progenitor genes. Sizes in base pairs for the corresponding marker bands (M) are shown on the left, adjacent to the gel images. PCR reactions performed with cDNA template are shown in lanes marked as '+' and negative control reactions performed with templates from the RT where reverse transcriptase was omitted are shown in lanes marked as '–'. Amplicons for a housekeeping gene (HPRT) under the same conditions are shown in the bottom panels.