Table 1 of
Reis, Mol Vis 2011; 17:2527-2532. Table 1 of
Reis, Mol Vis 2011; 17:2527-2532.
Table 1. Primer sequences and conditions for amplification of VSX2 exons.
| Set/exon | Forward primer sequence | Reverse primer sequence | PCR product | Annealing temp/ special conditions |
|---|---|---|---|---|
| Set 1/exon 1 | TCCAGAGCATTAGACACCGG | TGGCAGGAACTTTTCCGCCT | 603 bp | 55 °C (5% DMSO; 20% Betaine) |
| Set 2/exon 2 | GTTCAAAACCTCCGGATTCG | TCCGTTGTCGGCGAAAATAG | 392 bp | 55 °C |
| Set 3/exon 3 | TCTTGTCTGAGACAGGCTCT | TCATGGGCATCTGGAACCCT | 268 bp | 55 °C (5% DMSO; 20% Betaine) |
| Set 4/exon 4 | CACCATGGAGTAGGCGAGCT | ATTTCTCTCCTGCTAGGCTG | 432 bp | 55 °C |
| Set 5/exon 5 | CAGTTCAAGATGGCTTTCCC | ATGTCTCAGCATGGTCCAGA | 574 bp | 55 °C |