Figure 8. Apoptosis by TUNEL assay in each group at 28 days post-op. A-D: Immunohistochemical analysis for apoptosis by TUNEL assay. A: Group A, B: Group B, C: Group C, D: Group D. E: Quantitation of the number of apoptotic fibroblasts in the subconjunctival space at post-op 28 days. *p<0.01 for treated (rapamycin) versus control (Group B and D; n=4). Original magnification: 200×.