Table 1 of
De Bonis, Mol Vis 2011; 17:2482-2494. Table 1 of
De Bonis, Mol Vis 2011; 17:2482-2494.
Table 1. Primers used for LOX, SPARC, TIMP3, and SOD1 amplification.
| Primer name | Sequence | TM (°C) | Product size |
|---|---|---|---|
| VSX1_1F | 5’-CAGCTGATTGGAGCCCTTC-3’ | 58 | 599 |
| VSX1_1R | 5’-CTCAGAGCCTAGGGGACAGG-3’ | ||
| VSX1_2F | 5’-GCACTAAAAATGCTGGCTCA-3’ | 59 | 393 |
| VSX1_2R | 5’-GCCTCCTAGGAACTGCAGAA-3’ | ||
| VSX1_3F | 5’-CATTCAGAGGTGGGGTGTT-3’ | 59 | 419 |
| VSX1_3R | 5’-TCTTGTGGTGCCTTCAGCTA-3’ | ||
| VSX1_4F | 5’-CGTTGCTTTGCTTTGGAAAT-3’ | 59 | 394 |
| VSX1_4R | 5’-CGTTGCTTTGCTTTGGAAAT-3’ | ||
| VSX1_5F | 5’-CCCCAGAGATAGGCACTGAC-3’ | 59 | 495 |
| VSX1_5R | 5’-TGGACAATTTTTGTCTTTTGG-3’ | ||
| SPARC_2F | 5’-TTGCACATATCAGGAATTCAG-3’ | 58 | 250 |
| SPARC_2R | 5’-AGTCCCTGTTCCCTTTCAG-3’ | ||
| SPARC_3F | 5’-AAGCTCCCCTAGCCTGTATC-3’ | 60 | 250 |
| SPARC_3R | 5’-TAGCATTGAGACCCACAGC-3’ | ||
| SPARC_4F | 5’-ACCTGGAACCCTTCAGCTA-3’ | 58 | 248 |
| SPARC_4R | 5’-CTCATGTAGGCTGTCCTCGT-3’ | ||
| SPARC_5F | 5’-CCCTGAGATCTGTCCAGGTA-3’ | 58 | 294 |
| SPARC_5R | 5’-ATAGAACCACCAAGCCAACA-3’ | ||
| SPARC_6F | 5’-CAGTGTCCCCATCTCTGAA-3’ | 58 | 290 |
| SPARC_6R | 5’-GGTGGCAGAGACAGCATC-3’ | ||
| SPARC_7F | 5’-ACCAATGCAGGTGGTATGT-3’ | 58 | 348 |
| SPARC_7R | 5’-GCTCAGGGGTAAATGCAC-3’ | ||
| SPARC_8F | 5’-CATGGACCTCTTGTCACACA-3’ | 58 | 285 |
| SPARC_8R | 5’-AGGGCTTGGAGCAGTATAGG-3’ | ||
| SPARC_9F | 5’-AAATATCCTTTCCTCCATGCT-3’ | 58 | 377 |
| SPARC_9R | 5’-AGGCAGAGAGGACAGACAAC-3’ | ||
| SPARC_10F | 5’-TTGCATGGCCACCTAGAC-3’ | 58 | 246 |
| SPARC_10R | 5’-CTCCAGGCAGAACAACAAAC-3’ | ||
| SOD1_2F | 5’-CAGAAACTCTCTCCAACTTTGC-3’ | 59 | 218 |
| SOD1_2R | 5’-GAGGGGTTTTAACGTTTAGGG-3’ | ||
| LOX_1AF | 5’-GAGACTGAGATACCCGTGCT-3’ | 62 | 474 |
| LOX_1AR | 5’-AGCGGTGACTCCAGATGA-3’ | ||
| LOX_1BF | 5’-TCACAGTACCAGCCTCAGC-3’ | 62 | 500 |
| LOX_1BR | 5’-ATAGCTGGGGACCAGGTG-3’ | ||
| LOX_2F | 5’-TTTTCACATTGCTTTGCAGT-3’ | 56 | 398 |
| LOX_2R | 5’-GCTCTTGTCCCACTTCCTAA-3’ | ||
| LOX_3F | 5’-TAGTTGGGAAAGGAGGATTG-3’ | 58 | 355 |
| LOX_3R | 5’-GCAATTTTCTCCCTTCAGGT-3’ | ||
| LOX_4F | 5’-GACTTATGTCCTGGGGAAAA-3’ | 56 | 441 |
| LOX_4R | 5’-GATAAAAATGTGTGTGCTCTTCA-3’ | ||
| LOX_5F | 5’-GGAGGTGCTATAAGGCTGAG-3’ | 58 | 370 |
| LOX_5R | 5’-TTGCTTCCAATACCATGATT-3’ | ||
| LOX_6F | 5’-TTCAGGGGAAAATATGCAGT-3’ | 56 | 394 |
| LOX_6R | 5’-TGCTTACAAGAAAGCTGCTG-3’ | ||
| LOX_7AF | 5’-CTTAGGTGGAGGGAAACTGT-3’ | 58 | 485 |
| LOX_7AR | 5’-AAGTCATTTTGGCTCATTCA-3’ | ||
| LOX_7BF | 5’-GCACATAACTGGATTTTGAACG-3’ | 56 | 343 |
| LOX_7BR | 5’-TCAGCACCAGATGTGTCCAT-3’ | ||
| TIMP3_PrF | 5’-AGGGGTAGCAGTTAGCATTC-3’ | 60 | 516 |
| TIMP3_PrR | 5’-AGGAGGAGGAGAAGCCGT-3’ | ||
| TIMP3_1F | 5’-ACGGCAACTTTGGAGAGG-3’ | 60 | 273 |
| TIMP3_1R | 5’-GGGGCAGAGGAAAGGAGT-3’ | ||
| TIMP3_2F | 5’-CAATTCCAGGCTCCACAGAG-3’ | 60 | 300 |
| TIMP3_2R | 5’-CTGGCTGGTGCTTAGACACA-3’ | ||
| TIMP3_3F | 5’-ACATACCCAGCAGTGGGATT-3’ | 60 | 296 |
| TIMP3_3R | 5’-ACTGGACATTTGGTGAGTCAA-3’ | ||
| TIMP3_4F | 5’-GGCTAGGCTCTGGACAAAAC-3’ | 56 | 248 |
| TIMP3_4R | 5’-GCATTGGGAGCTGATGTTTC-3’ | ||
| TIMP3_5F | 5’-GTCTGAATCCAGGCTCGGTA-3’ | 56 | 387 |
| TIMP3_5R | 5’-TTTGCAAGAAAACAGGCACT-3’ |