Figure 5. Mitochondrial membrane potential (MMP) and cell apoptosis assay. A: Summarized data for MMP change for shRNA treated RCE cells. The ratio of JC-1 red/green fluorescence represents the MMP. y-axis is the ratio of JC-1 red to green, which reflects MMP. Data are normalized with no treatment. MMP for shRNA treated cells relative to that of no treatment (NT). B: Summarized data from the Annexin V-FITC (AnV) cell apoptosis assay results. Annexin V-FITC intensity was normalized to protein concentration.