Figure 4. ROS production assay. A: MitoSOX™ microscopy ROS detection for siRNA treated RCE cells (200×). B: A representative histogram of ROS production for control, scrambled virus-treated and shRNA-treated cells with and without 2 min UV exposure. An increase in relative fluorescence is reflected with a rightwards shift in x-axis in line histograms. The % of Max is the number of cells in each bin divided by the number of cells in the bin that contains the largest number of cells. C: Summary data for DCF flow cytometry ROS detection.