Figure 1. Expression levels of Tyrp1
across strains. A: Rank ordered mean of Tyrp1
mRNA expression levels across 67 BXD RI strains, their parental
strains and F1 crosses. Values denote normalized relative
expression levels on a log2 scale (mean±SEM). The majority of
lines with the Tyrp1b mutation expressed
higher levels of Tyrp1 than those with the wildtype
allele. F1 and parental strains had intermediate mRNA expression
levels. B: western blot showing Tyrp1 protein levels in
eyes and irides of parental strains. Tyrp1 protein levels were
normalized to Gapdh and the amount of protein in eyes of B6 mice
was set to 1. There is no statistical difference in Tyrp1
protein levels between strains with wildtype versus mutant Tyrp1.