Figure 6. Corneal incision induces
the death of epithelial and stromal cells shortly after injury.
Transverse sections of the cornea were labeled for TUNEL (A-F),
DAPI (I, L, N, and O), or EtHD (J,
M, N, and O). Corneas were obtained at 15
min (G and K-O), 2 h (B), 4 h (C),
1 day (D), 4 days (E) or 7 days (F) after
injury. The green lines indicate Bowman’s membrane and the edge
of the incision. Panel G is a whole-mount preparation of
an acutely injured cornea that was labeled with EtHD. Panels H
and K are DIC images of control and damaged corneas.
Panels N and O are 2.5 fold enlargements of the
boxed-out (yellow rectangles) areas in panel K. Arrows
indicate the nuclei of dying cells. The calibration bar (50 µm)
in panel F applies to panels A-F, the bar
in panel in G applies to G alone and the bar in
panel M applies to panels H-M.
Abbreviations: epi – epithelium, str – stroma, endo –