Figure 4. CD45+ monocytes accumulate in the stroma following corneal injury. Transverse section of undamaged (A and B) and injured (C-L) corneas were labeled with Draq5 (red nuclei in A-I; blue nuclei J and L) and antibodies to CD45 (green) and BrdU (red in J and L). Corneas were harvest at 1 (C, G, and J), 4 (D, E, and L), 7 (F), and 14 (H and I) days after injury. Panel G is a 2.5 fold enlargement of the area (yellow box) in C indicating the amoeboid morphology of CD45+ monocytes in the site of injury in the stroma. Panel K is a 1.5 fold enlargement of the area (yellow box) in J providing a single-channel images and a merged overlay of labeling for CD45, BrdU and Draq5 in a single monocyte. Arrows indicate cells labeled for CD45 and Draq5, arrowheads indicate BrdU-labeled cells (J and L), and small double-arrows indicate a single cell labeled for CD45, BrdU and Draq5 (J and K). The calibration bar (50 µm) in panel A applies to A alone, the bar in I applies to B-I, and the bar in L applies to J and L. Abbreviations: epi – epithelium, str – stroma, endo – endothelium.