Table 1 of
Asomugha, Mol Vis 2011; 17:2407-2420. Table 1 of
Asomugha, Mol Vis 2011; 17:2407-2420.
Table 1. Oligonucleotide primers used for subcloning WT αB-crystallin and deamidated αB species, and generating truncated αB mutant proteins using PCR-based deletion.
| Mutant constructs | Direction | Primers (5′–3′) |
|---|---|---|
| WT αB | forward | CACCATGGACATCGCCATCCACCACCCCTG |
| reverse | CTATTTCTTGGGGGCTGCGGTGACAGC | |
| αB N146D | forward | CACCATGGACATCGCCATCCACCACCCCTG |
| reverse | CTATTTCTTGGGGGCTGCGGTGACAGC | |
| αB-NT | forward | CACCCGCCTGGAAAAGGACAGGTTCTCTG |
| reverse | CTATTTCTTGGGGGCTGCGGTGACAGC | |
| αBN146D-NT | forward | CACCCGCCTGGAAAAGGACAGGTTCTCTG |
| reverse | CTATTTCTTGGGGGCTGCGGTGACAGC | |
| αB-CT | forward | CACCATGGACATCGCCATCCACCACCCCTG |
| reverse | TTATTTCCTTGGTCCATTCACAGTGAG | |
| αBN146D-CT | forward | CACCATGGACATCGCCATCCACCACCCCTG |
| reverse | TTATTTCCTTGGTCCATTCACAGTGAG |