Figure 3. Far-UV CD spectra of WT αB-crystallin and its mutant proteins. Spectra were recorded using protein preparations of 0.2 mg/ml, dissolved in 50 mM sodium phosphate buffer (pH 7.8), and a cell path length of 0.5 mm. The reported spectra are the average of 5 scans corrected for the buffer blank and smoothed. A: WT αB-crystallin and deamidated mutant. B: WT αB-crystallin and NH₂-terminal domain deleted mutants. C: WT αB-crystallin and COOH-terminal extension deleted mutants.