Figure 2. SDS–PAGE analysis of purified, His-tagged WT αB-crystallin and its deamidated, NH2- or COOH-terminally deleted mutants, and deamidated plus deleted mutants, following Ni2+-affinity column purification (see Methods). Lane 1 – molecular weight marker; Lane 2 – WT αB-crystallin; Lane 3 – αB N146D;
Lane 4 – αB-NT; Lane 5 – αB N146D-NT; Lane 6 – αB-CT; αB N146D-CT.