Figure 1. Schematic diagram showing the regions and residue numbers of the NH₂-terminal domain, α-crystallin domain, COOH-terminal extension and deamidation sites in WT αB-crystallin and its mutants. WT αB-crystallin is a full-length protein containing all residues from 1 to 175. Residue N146 was deamidated (i.e., N to D) in the deamidated mutants. NH₂-terminal domain deleted mutants (αB-NT and αB N146D-NT) are missing residues no. 1–66, while COOH-terminal extension deleted mutants (αB-CT and αB N146D-CT) are missing residues no. 151–175.