Molecular Vision: Khan, Mol Vis 2011; 17:218-224. Table 1

Table 1. Primers for KIF21A.

Exon	5′ to 3′ primer sequence	PCR conditions
Kfi21a_x1Fn	ctgttggcttctccacagg	52 °C/35 cycles
Kfi21a_x1Rn	gggactcactgcctcagttt
Kfi21a_x2Fn	tcatgattttgggggattgt	53 °C/35 cycles
Kfi21a_x2Rn	caaaaatgaaagcgcaactg
Kif21a_x3F	tcagttgcgctttcatttttg	53 °C/35 cycles
Kif21a_x3R	ctccaacctgggtgacagaa
Kif21a _x4F	tagcctcattcattttaatgtgtt	59 °C/35 cycles
Kif21a _x4R	gatcttaattccatgtcatgcttc
Kif21a _x5F	tgcctgtaactgaactaataatgtga	59 °C/35 cycles
Kif21a _x5R	atggctgaccagcttcaact
Kif21a _x6F	tttggctttatgcctgtttc	59 °C/35 cycles
Kif21a _x6R	tgaggagattggagattcagtg
Kif21a_x7F	cttatttctgtttcaaagaattagta	59 °C/35 cycles
Kif21a_x7R	cctacacctcaagggatgct
Kif21a _x8F	caggggcttttaaatttgct	59 °C/35 cycles
Kif21a _x8R	ctccaaaaggaaggaggaca
Kif21a_x9Fn	tggtcttgaactcctgacctc	59 °C/ 35 cycles
Kif21a_x9rn	tgccctccagaagttaatcc
Kif21a_x10F	tgtggtctgctcatgtaataaagg	53 °C/35 cycles
Kif21a_x10R	ggaatatgacatcaagggaaagg
Kif21a_x11Fn	ccacagagaaaaatgctcccta	59 °C/35 cycles
Kif21a_x11Rn	tgaatggaatgcaaaagcag
Kif21a _x12F	gcatccaagcatgcctaatc	59 °C/35 cycles
Kif21a _x13R	tttaggagcagcccagctta
Kif21a_x13Fn	tgattggcaatttccattttt	59 °C/35 cycles
Kif21a_x13Rn	gactccccaacacaatgctt
Kif21a _x14F	gttggggagtcaggggtaga	56 °C/35 cycles
Kif21a _x14R	taaagccttggaaggcaaatg
Kif21a _x15F	cattcaccttttggttgttgg	59 °C/35 cycles
Kif21a _x15R	aggcacaaactttgacttgc
Kif21a _x16F	gacaccctagtcttctgagatgtg	59 °C/35 cycles
Kif21a _x16R	ttgccaaaggaaattacatca
Kif21a _x17F	taaacgtgcagcaaaactgc	59 °C/35 cycles
Kif21a _x17R	tgcttatctattgtccttaacctgc
Kif21a x18F	tggccgttaatactgaatgttg	56 °C/35cycles
Kif21a x18R	aaagcaggttggattttaagaaa
Kif21a_x19F	ccatttggaagaaaccttctg	56 °C/35 cycles
Kif21a_x19R	tgcactgccaaataatgagc
Kif21a _x20–21F	ggcaacaaatggaaacaggt	59 °C/35 cycles
Kif21a _x20–21R	tggcatacatgtaaaacctaagc
Kif21a _x22F	ccctatgtttcttggggtaatgat	59 °C/35 cycles
Kif21a _x22R	tccttattacaaagcaaagggtta
Kif21a _x23–24F	ttactggaggagctgggatg	59 °C/35 cycles
Kif21a _x23–24R	tagtgtgtttgtgggcatgg
Kif21a _x25_ 26F	actaaaaccatcgtgcccat	59 °C/35 cycles
Kif21a _x25_ 26R	gctttagtaaaaccatgccctc
Kif21a 26F	tggcctagtgaatagcacttagaa	59 °C/35 cycles
Kif21a 26R	cagttaccacttaaagggaaatatga
Kif21a _x27F	cacacctaggaaaagacacgct	56 °C/35 cycles
Kif21a _x27R	ggggagacaacacctagcaa
Kif21a_x28F	caagtaataatctttctgaggttcca	56 °C/35 cycles
Kif21a_x28R	accacagcaccagcctaaat
Kif21a _x29F	ttgttcagaatgcattttatcttaca	59 °C/35 cycles
Kif21a _x29R	gcatggttcctttcccatt
Kif21a _x30F	agcagggcactatgaaggaa	56 °C/35 cycles
Kif21a _x30R	tttatctaaaaggtatgaccacaaaa
Kif21a_x31Fn	tgtctcattccctttcacca	56 °C/35 cycles
Kif21a_x31Rn	caacagacttgatctgaaggaga
Kif21a _x32F	gcttaaaagagagcagtfctgga	59 °C/35 cycles
Kif21a _x32R	ggttgaaccagattatccga
Kif21a _x33F	tgaagttaggatccttgtggtatg	59 °C/35 cycles
Kif21a _x33R	tgggaagtggacaggtatacaa
Kif21a _x34F	tgtgttaggtgctgtgctagg	56 °C/35 cycles
Kif21a _x34R	aaggacacaagagacatttagagg
Kif21a _x35F	gcccaagatcccatctctaa	56 °C/35 cycles
Kif21a _x35R	ccactaactatgaatgaaggaaaaga
Kif21a_x36Fn	ctccagcctgggaaacatag	59 °C/35 cycles
Kif21a_x36Rn	ggcctgattaatattatctgtaaatga
Kif21a _x37F	ctttctccagccaattccaa	59 °C/35cycles
Kif21a _x37R	aacctggggtgcctaaattc
Kif21a _x38F	tgtaaagggcacatggtaacaa	59 °C/35 cycles
Kif21a _x38R	gcagttgaattcagatatattttcca