Figure 2. Real-time RT–PCR of β
subunits from whole LG (left panels) and epithelial cells
collected by LCM (right panels). mRNA levels of β1 and β3 from
whole LG of IAD rabbits were significantly lower than control
animals (p<0.05), whereas no significant difference was
detected between control and IAD animals for β2 (p>0.05). β1:
In epithelial cells collected by LCM, β1 was most abundant in
acini, and their level in IAD appeared to be similar in duct
segments and was significantly lower than control animals [
13]
in acini and all duct segments (p<0.05). β2: mRNA was least
abundant in acini and most abundant in interlobar duct, and its
levels in IAD rabbits were significantly lower than control
animals in acini and all duct segments except in intralobar duct
(p<0.05). β3: mRNA was least abundant in acini while its
levels were similar in all duct segments. Compared to control
animals [
13],
its levels in IAD animals were significantly lower in acini
(p<0.05), while no differences were detected in duct segments
(p>0.05). D4: intralobular duct. D3: interlobular duct. D2:
intralobar duct. D1: interlobar duct. Data were presented as
mean±SEM.