Table 1 of
Asomugha, Mol Vis 2011; 17:2356-2367. Table 1 of
Asomugha, Mol Vis 2011; 17:2356-2367.
Table 1. Oligonucleotide primers used for generation of individual domain constructs of αA- and αB-crystallins using PCR-based deletion.
| Mutant constructs | Direction | Primers (5′-3′) |
|---|---|---|
| αA NTD | Fwd | CACCATGGACGTGACCATCCAGCACCCC |
| Rev | TTACTCAGAGATGCCGGAGTCCAGCACGGT | |
| αA CD | Fwd | CACCGTTCGATCCGACCGGGACAAGTTCGTC |
| Rev | TTAACAGAAGGTCAGCATGCCATCGGCAGA | |
| αA CTE | Fwd | CACCGGCCCCAAGATCCAGACTGGCCTGGAT |
| Rev | TTAGGACGAGGGAGCCGAGGTGGGGTTCTC | |
| αB NTD | Fwd | CACCATGGACATCGCCATCCACCACCCC |
| Rev | TTATGAGAGTCCAGTGTCAAACCAGCT | |
| αB CD | Fwd | CACCGAGATGCGCCTGGAAAAGGACAGG |
| Rev | TTAATTCACAGTGAGGACCCCATCAGA | |
| αB CTE | Fwd | CACCGGACCAAGGAAAGAGGTCTCTGGC |
| Rev | CTATTTCTTGGGGGCTGCGGTGAC |