Figure 4 of Asomugha, Mol Vis 2011; 17:2356-2367.


Figure 4. Fluorescence spectra of WT αA, WT αB and their individual domain constructs following ANS binding. Fluorescence spectra were recorded by excitation at 390 nm and emission from 400 to 600 nm, with protein preparations (0.2 mg/ml) mixed with 15 μl of 0.8 mM ANS (dissolved in methanol) and incubated at 37 °C for 15 min. A: WT αA, NH2-terminal domain, and core domain constructs. B: WT αB, NH2-terminal domain, core domain, and COOH-terminal extension constructs. Dotted lines indicate the maximum peak wavelength (λmax) of the respective WT α-crystallins, used to determine whether a blue or red shift in wavelength occurred.