Figure 3 of Nowińska, Mol Vis 2011; 17:2333-2342.


Figure 3. Representative images of histopathologic analysis of four corneal sections – three after penetrating keratoplasty and one after deep anterior lamellar keratoplasty. A: Section of the cornea after deep anterior lamellar keratoplasty. Male patient (F1; 37 years old). Green birefringence is visible with a polarizing filter (arrowheads). Stromal deposition of amyloid substance in anterior corneal part distorts the architecture of the corneal lamellae. The absence of Bowman’s layer and thinning of the epithelium are noticeable. LCDI/R124C mutation. B: Section of the cornea after penetrating keratoplasty. Congo red stain. Female patient (F2; 45 years). Deposits throughout the corneal stroma stain positive with Congo red. Note the deep, posterior corneal location of the deposit (arrowhead). LCD variant/H626 mutation. C: Section of the cornea after penetrating keratoplasty. PAS stain. Female patient (F4; 53 years). Note the absence of Bowman’s layer and the distorted epithelium in correspondence of the granular deposits (arrowheads). There are several granular deposits throughout the corneal stroma (arrows). GCDI/R555W mutation. D: Section of the cornea after penetrating keratoplasty. PAS stain. Female patient (F4; 53 years). Masson trichrome stain. Section of the cornea showing the absence of Bowman’s layer and the absence of the epithelium in correspondence with the Masson trichrome – positive granular deposits (arrows). GCDI/R555W mutation. E: Section of the cornea after penetrating keratoplasty. Masson trichrome stain. Female patient (F9; 44 years). Note that the granular deposits are placed under the thinner epithelium, thus taking the place of the former Bowman’s layer. GCDII/R124H mutation. F: Section of the cornea after penetrating keratoplasty. Congo red stain. Female patient (F9; 44 years). Note the Congo red positive deposits in the anterior corneal stroma. GCDII/R124H mutation.