Figure 7. Effect of GSPE on H₂O₂-induced phosphorylation of the MAPK pathway. HLEB-3 cells were incubated with 20 mg/l GSPE for 12 h. Cells were harvested at 1, 2, and 3 h time points after treated with 100 μmol/l H₂O₂, and p38 and JNK activities were evaluated by western blotting. GSPE markedly reduced H₂O₂induced phosphorylation of p38 and JNK at each time point studied. The total amount of JNK and p38 remained unchanged at each time point studied.