Table 1 of
Low, Mol Vis 2011; 17:2272-2282. Table 1 of
Low, Mol Vis 2011; 17:2272-2282.
Table 1. Primer and annealing conditions for bestrophin-1 (BEST1) sequencing.
| Annealing temperature (°C) | Additional information | Primer name | Sequence 5′-3′ |
|---|---|---|---|
| Exon 2F | CACCTGCTGCAGCCCACTGCC | ||
| 61 | Exon 2R | CTTGTAGTGAACTGGTACACTGGCC | |
| Exon 3F | GGACAGTCTCAGCCATCTCCTCG | ||
| 59 | Exon 3R | GCAGCTCCTCGTGATCCTCCCCTGG | |
| Exon 4F | CTAGGCCCGCTCGCAGCAGAAAGC | ||
| 60 | 10% DMSO | Exon 4R | CTTCCATTCCTGCCGCGCCCATCTC |
| Exon 5/6F | CATCCCTTCTGCAGGTTCTC | ||
| 59 | Exon 5/6R | CTTGGTCCTTCTAGCCTCAGTTTC | |
| Exon 7F | CTGGAGCATCCTGATTTCAGGGTTC | ||
| 59 | Exon 7R | CTCTGGCCATGCCTCCAGC | |
| Exon 8/9F | GCTGGCTTTGAGGAGTTCTGCCTG | ||
| 59 | Exon 8/9R | GTGCTATTCTAAGTTCCTAGGCAG | |
| Exon 10F | GTAAGGGAGAAGTAAGGCCAGGTG | ||
| 59 | Exon 10 R | GTAGGTCCAGTGTGCTCTGGCAG | |
| Exon 11F | GAAGGGACCTTCCATACTTATG | ||
| 59 | Exon 11R | CATTAAAGGCTGAAGTAGTCTGGG |