Table 1 of
Li, Mol Vis 2011; 17:2263-2271. Table 1 of
Li, Mol Vis 2011; 17:2263-2271.
Table 1. Primers used for S100A qPCR.
Gene name |
Primer sequences (5′-3′) |
Amplicon size (bp) |
Annealing temperature |
|---|---|---|---|
| S100A1 | F: CTTGGCCATCTGTCCAGAAC | ||
| R: CAATGTGGCTGTCTGCTCAA | 362 | 56 °C | |
| S100A2 | F: GCCAAGAGGGCGACAAGTT | ||
| R: AGGAAAACAGCATACTCCTGGA | 175 | 60 °C | |
| S100A3 | F: GCGGTAGCTGCCATCGTGTG | ||
| R: GCAGTCCTTGAAGTACTCGT | 258 | 56 °C | |
| S100A4 | F: GATGAGCAACTTGGACAGCAA | ||
| R: CTGGGCTGCTTATCTGGGAAG | 123 | 60 °C | |
| S100A5 | F: CTGCACACTGTGATGGAGAC | ||
| R: GAAGTCGTTGTAGGCCATGC | 270 | 56 °C | |
| S100A6 | F: AAGCTGCAGGATGCTGAAAT | ||
| R: CCCTTGAGGGCTTCATTGTA | 131 | 56 °C | |
| S100A7 | F: TGCTGACGATGATGAAGGAG | ||
| R: ATGTCTCCCAGCAAGGACAG | 131 | 56 °C | |
| S100A8 | F: ATGCCGTCTACAGGGATGAC | ||
| R: ACGCCCATCTTTATCACCAG | 160 | 56 °C | |
| S100A9 | F: GTGCGAAAAGATCTGCAAAA | ||
| R: TCAGCTGCTTGTCTGCATTT | 103 | 56 °C | |
| S100A10 | F: ATGCCATCTCAAATGGAACA | ||
| R: CTACTTCTTTCCCTTCTGCT | 294 | 56 °C | |
| S100A11 | F: ATGGCAAAAATCTCCAGCCC | ||
| R: TCATCATGCGGTCAAGGACA | 193 | 56 °C | |
| S100A12 | F: CCTCTCTAAGGGTGAGCTGA | ||
| R: CTGGGTTTTGGTGAGGGAAA | 271 | 56 °C | |
| ACTB | F: ATCATGTTTGAGACCTTCAACA | ||
| R: CATCTCTTGCTCGAAGTCCA | 318 | 56 °C | |
| GAPDH | F: CCATGTTCGTCATGGGTGTGAACGA | ||
| R: GCCAGTAGAGGCAGGGATGATGTTC | 254 | 56 °C |