Figure 4. S100A2, S100A10, S100A8, and S100A9 mRNA expression in cultured limbal epithelial cells. A: Fold changes of the above mRNA in P1, P3, and P5 limbal epithelial cells. mRNA level in P1 limbal cells was set as 100%. B: Fold changes of the above mRNA in limbal epithelial cells cultured in serum- and growth hormone-depleted medium. mRNA level in cells grown in full medium was set as 100%. C: western blot analysis of CK3 protein in P1, P3, and P5 limbal epithelial cells.