Table 1 of
Kinori, Mol Vis 2011; 17:2241-2247. Table 1 of
Kinori, Mol Vis 2011; 17:2241-2247.
Table 1. Primers used to amplify candidate genes for the new retinal phenotype demonstrating enhanced S-cone function with preserved rod function: the nuclear receptor subfamily 2 group E member 3 (NR2E3), neural retina leucine zipper (NRL), thyroid hormone receptor beta (THRB) and nuclear receptor subfamily 1 group D member 1 (NR1D1) genes.
| Primer name | Gene | Exon | Sequence 5′-3′ | PCR product size |
|---|---|---|---|---|
| NR2E3–1F | NR2E3 | 1 | CAGGCCTCCCGCAGGCAGGCAG | 242 |
| NR2E3–1R | NR2E3 | 1 | CCATGGTCCCTGCGAACCTC | |
| NR2E3–2F | NR2E3 | 2 | GAGGGGAGCGTGCAGCCCTG | 248 |
| NR2E3–2R | NR2E3 | 2 | CACCCCTCCAGAACCCCTCAG | |
| NR2E3–3F | NR2E3 | 3 | GTCCAAGCCCATGGCTCAGG | 242 |
| NR2E3–3R | NR2E3 | 3 | GAAGGGTCAGGACGACACGC | |
| NR2E3–4F | NR2E3 | 4 | GGGAGGTGACAAGAAATGGGC | 327 |
| NR2E3–4R | NR2E3 | 4 | GAAGCCAAGCCCTGCTGTGC | |
| NR2E3–5F | NR2E3 | 5 | CAAGTACTCCCTGCCACCTC | 316 |
| NR2E3–5R | NR2E3 | 5 | GTGCCCTGTCTGGTTGACTC | |
| NR2E3–6F | NR2E3 | 6 | GCTGTGTGTCTGCCATAACA | 316 |
| NR2E3–6R | NR2E3 | 6 | CTCAAGGTTTGGGCAGAGAC | |
| NR2E3–7F | NR2E3 | 7 | CTGTGCTAAGCTCACTGGTG | 183 |
| NR2E3–7R | NR2E3 | 7 | GAGGTCAGGGACAGATGAGTG | |
| NR2E3–8F | NR2E3 | 8 | GTCGTAAAACTGATGGCGTCCTC | 232 |
| NR2E3–8R | NR2E3 | 8 | GCAAATGTTTCGTTTCAGTAGATTG | |
| NRL-1F | NRL | 1 | CACAGATGACCTCAGAGAGCTGGCCCTTTA | 237 |
| NRL-1R | NRL | 1 | CAGGTGTTAAAGAGGGGGTTCTAGGTGAGC | |
| NRL-2F | NRL | 2 | ACCATCCCTCTGGCTTTCCAAACTCTTGCT | 683 |
| MRL-2R | NRL | 2 | GATCTGATTGCTTTCAAGGGACCTTCTCCC | |
| NRL-3F | NRL | 3 | GACCTGGCGCTGACCCGGTTTCTGCATTCT | 429 |
| NRL-3R | NRL | 3 | GCCACCCCCACCAGCCCCCACTACACCACA | |
| THRB-1F | THRB | 1 | TGGAGAATGCATGCGTAGAC | 439 |
| THRB-1R | THRB | 1 | CGAAAACAAACAGTGAAACTTTG | |
| THRB-4F | THRB | 4 | AATGCATATGATATTGTTTGGAAC | 264 |
| THRB-4R | THRB | 4 | GGTTTGGAAATAACGGTTGC | |
| THRB-5F | THRB | 5 | CTTGCCTTCCAAAACTCTGC | 261 |
| THRB-5R | THRB | 5 | CACCATACATTGGAAGAGAAATG | |
| THRB-6F | THRB | 6 | TCTGGCCTAGCAACCTTAGC | 101 |
| THRB-6R | THRB | 6 | ACTGGGAGGGGACTGGAG | |
| THRB-7F | THRB | 7 | AGGTCCAAAACGATTCATCTC | 148 |
| THRB-7R | THRB | 7 | CCCAGTCGATCTCCTTGAAC | |
| THRB-8F | THRB | 8 | TTGCTGTGTATCTTGGGAGC | 206 |
| THRB-8R | THRB | 8 | TCCCAAGGTGATGAGGACTG | |
| THRB-9F | THRB | 9 | TTCAGAAGAGATTTTCTGCCAC | 147 |
| THRB-9R | THRB | 9 | TCGTTTTGTACTGACGTTGC | |
| THRB-10F | THRB | 10 | CAAATGTTAATCACAGAAGGTTATTCC | 259 |
| THRB-10R | THRB | 10 | AGCGCTAGACAAGCAAAAGC | |
| THRB-11F | THRB | 11 | ATTGGACAAAGCAAGCCTTC | 242 |
| THRB-11R | THRB | 11 | TGGAATGAAATGACACCCAG | |
| NR1D1–1F | NR1D1 | 1 | TCTCTCTGCTCTTCCCATGC | 361 |
| NR1D1–1R | NR1D1 | 1 | CACCCCAGTCCCTTACAAAG | |
| NR1D1–2F | NR1D1 | 2 | GCTCCACATGGTGAACTGAG | 553 |
| NR1D1–2R | NR1D1 | 2 | GTCCTGGCAAGACTGGTGTC | |
| NR1D1–3–4F | NR1D1 | 40606 | CTTTTCCCTCCCTGGATCTC | 532 |
| NR1D1–3–4R | NR1D1 | 40606 | CAGTATGATGTGTCTCCATTTGTG | |
| NR1D1–5F | NR1D1 | 5 | GTGAAACCCCAAGCCTTCAG | 850 |
| NR1D1–5F | NR1D1 | 5 | CACACTCAGCCTCCAGGAAC | |
| NR1D1–6F | NR1D1 | 6 | CATCCTTCAGGGCCACAG | 390 |
| NR1D1–6F | NR1D1 | 6 | AGATCGCACCATTGCACTC | |
| NR1D1–7F | NR1D1 | 7 | TTCAGAAACAACCCCCACTC | 345 |
| NR1D1–7F | NR1D1 | 7 | CCTACCTGCAGAGACAAGCAC | |
| NR1D1–8F | NR1D1 | 8 | CAGGGAGAGGCTGCATTG | 392 |
| NR1D1–8R | NR1D1 | 8 | TGGTTTGCTTTTCCTTTTCG |