Figure 7. PKR associated protein X (RAX) expression in the presence of a microRNA-29b mimic. The expression of RAX protein was evaluated by western blot analysis. A: The expression of RAX protein in CJ4 cells transfected with a miR-29b mimic at a concentration of 0, 50, or 75 nM after 24 and 48 h of transfection. B: The expression of RAX protein in CJ4 cells transfected with dsRNA (negative control) at a concentration of 0, 50, or 75 nM, 24 and 48 h after transfection. β-actin was used as a loading control. Rats were used in groups of three. Data are means±standard error of the mean from three independent experiments, and each sample was run in six replicates. The immunoblot is representative of one typical experiment.