Figure 5 of Silva, Mol Vis 2011; 17:2228-2240.


Figure 5. PKR associated protein X (RAX) expression in the retinas of rats after treatment with streptozotocin. A: RAX mRNA expression was evaluated by qRT–PCR and was normalized to ACTB. B: RAX protein expression was evaluated by western blot using anti-RAX antibody. Rats were used in groups of three. Data are means±standard error of the mean from three independent experiments, and each sample was run in six replicates. C: The immunoblot is representative of one typical experiment. For both the mRNA and the protein levels of RAX, the paired Student t test was performed to ascertain whether there is statistical significance between the retinas of diabetic rats and nondiabetic rats. The asterisks (*, ** and ***) indicate statistical significance (p<0.05, p<0.005 and p<0.0005, respectively) between control and experimental groups.