Figure 5. PKR associated protein X (RAX)
expression in the retinas of rats after treatment with
streptozotocin. A: RAX mRNA expression was
evaluated by qRT–PCR and was normalized to ACTB. B:
RAX protein expression was evaluated by western blot using
anti-RAX antibody. Rats were used in groups of three. Data are
means±standard error of the mean from three independent
experiments, and each sample was run in six replicates. C:
The immunoblot is representative of one typical experiment. For
both the mRNA and the protein levels of RAX, the paired Student
t test was performed to ascertain whether there is
statistical significance between the retinas of diabetic rats
and nondiabetic rats. The asterisks (*, ** and ***) indicate
statistical significance (p<0.05, p<0.005 and p<0.0005,
respectively) between control and experimental groups.