Figure 6. Actin (green) and Nuclei (red) staining of the endothelium in normal C57Bl/6 and Ctns−/− mice. A: The endothelium of a 12 month old normal C57Bl/6 mouse, with the typical hexagonal shape and regular size. B: The endothelium of a 12 month old Ctns−/− cornea. We observed an evident increased cell size with polymorphism, polymegathism, and multinucleation.