Figure 5. Corneal staining with actin and nuclei staining. A: Actin (Green) and nuclei (red) staining of cornea in normal C57Bl/6 mouse using confocal microscopy. We observed normal
keratocytes cell bodies with dendritic morphology that stained in the periphery for actin. B: Actin and nuclei staining of corneas from Ctns−/− mice at 5 months of age showing few inflammatory cells (arrow). Inflammatory cell infiltration appeared to increase in the
10th (C, arrows) and 12th (D, arrows) month of age with keratocyte dropout (asterisk).