Figure 4 of VanderWyst, Mol Vis 2011; 17:199-209.


Figure 4. Integrin α6 subunit expression by Schlemm’s canal cells. A: Confocal immunofluorescence microscopy of outflow tissues including Schlemm’s canal (SC) from human donor eyes showing α6 integrin (INTG) levels. Shown is representative image from eye of individual human donor of 3 donor eyes that were examined. For comparison, a scleral vessel is indicated (*). B: western blot analysis of different human SC and trabecular meshwork (TM) cell strains checking for α6 integrin levels. Cell lysates were first subjected to immunoprecipition using integrin α6-specific IgGs, followed by SDS–PAGE and western blotting using IgGs that recognize both α3 and α6 INTG.