Figure 1. Collagens in human conventional outflow pathway and cultured cells. A: Confocal immunofluorescence microscopy of outflow tissues from cadaveric human eyes examining the expression levels of collagen (CN) I, III, and IV. Vascular endothelial cadherin (VE-cad) labeling was used as positive control for integrity of tissue antigens and localization of Schlemm’s canal (SC). Shown are representative images taken from section of one donor eye of 3 examined. B: Western blot analysis of collagen levels in different strains of cultured human trabecular meshwork (TM) and SC cell monolayers isolated from different individual eye donors. Anti-collagen I IgGs recognized multiple bands between 140 and 40 kDa, corresponding to post-translational modifications.