Table 1 of
de Figueirêdo, Mol Vis 2011; 17:2207-2211. Table 1 of
de Figueirêdo, Mol Vis 2011; 17:2207-2211.
Table 1. Primers for PCR amplification of the CRYAA, CRYGC, and CRYGD genes, product sizes, and annealing temperatures.
| Gene | Exon | Strand | Sequence (5′→3′) | Fragment Size (bp) | T (°C) |
|---|---|---|---|---|---|
| CRYAA | 1 | sense | CACGCCTTTCCAGAGAAATC | 466 | 63.9 |
| 1 | antisense | CTCTGCAAGGGGATGAAGTG | |||
| 2 | sense | CTTGGTGTGTGGGAGAAGAGG | 377 | 58.0 | |
| 2 | antisense | TCCCTCTCCCAGGGTTGAAG | |||
| 3 | sense | CCCCCTTCTGCAGTCAGT | 989 | 57.0 | |
| 3 | antisense | GCTTGAGCTCAGGAGAAGGA | |||
| CRYGC | 1 - 2 | sense | ACCAGAGAACAAGGACACAATC | 674 | 66.6 |
| 1 - 2 | antisense | TGGCTTATTCAGTCTCTGATG | |||
| 3 | sense | ATTCCATGCCACAACCTACC | 590 | 66.3 | |
| 3 | antisense | CCAACGTCTGAGGCTTGTTC | |||
| CRYGD | 1 - 2 | sense | CCCTTTTGTGCGGTTCTTG | 596 | 54.0 |
| 1 - 2 | antisense | TTTGTCCACTCTCAGTTATTGTGAC | |||
| 3 | sense | TGTGCTCGGTAATGAGGAG | 700 | 61.0 | |
| 3 | antisense | AGGCCAGAGAATCAAATGAG |