Table 1 of
Zhang, Mol Vis 2011; 17:2139-2147. Table 1 of
Zhang, Mol Vis 2011; 17:2139-2147.
Table 1. Primers used for amplification and sequencing of PAX6.
| Primer ID | Sequence (5′-3′) | Product length (bp) | Annealing Temperature (°C) |
|---|---|---|---|
| Extra-E1-F* | GAGCTGTGCCCAACTCTAGC | ||
| Extra E1-R | TCCATCTTTGTATGCCTCCTTT | 399 | 56 |
| Exon1F | GGAGAGGGAGCATCCAATC | ||
| Exon1R | TCCTGGGAAGGAGACAGAGA | 396 | 56 |
| Exon2F | ACACACTTGAGCCATCACCA | ||
| Exon2R | CTCCTGCGTGGAAACTTCTC | 467 | 56 |
| Exon3F | AGAGAGCCCATGGACGTATG | ||
| exon3R | CCCAATCTGTTTCCCCTACA | 318 | 56 |
| Exon4F | TGCAGCTGCCCGAGGATTA | ||
| Exon4R | GCACCCCGAGCCCGAAGTC | 144 | 66 |
| Exon5F | TCCCTCTTCTTCCTCTTCACT | ||
| Exon5R | GGGGTCCATAATTAGCATC | 301 | 61 |
| Exon6–7F# | GCTCTCTACAGTAAGTTCTC | ||
| Exon6–7R | AGGAGAGAGCATTGGGCTTA | 457 | 61 |
| Exon8F | GATTTGCAGGTGTCATCAAT | ||
| Exon8R | ATATGGAGAGCTGCGTGGAT | 212 | 65 |
| Exon9F | TTTGGTGAGGCTGTCGGGA | ||
| Exon9R | TCTTTGTACTGAAGATGTGGC | 339 | 58 |
| Exon10F | GTAGTTCTGGCACAATATGG | ||
| Exon10R | GTACTCTGTACAAGCACCTC | 206 | 62 |
| Exon11–12F | GGCTCGACGTAGACACAGT | ||
| Exon11–12R | TGCAGACACAGCCAATGAGG | 500 | 62 |
| Exon13F | GCTGTGTGATGTGTTCCTCA | ||
| Exon13R | AAGAGAGATCGCCTCTGTG | 245 | 62 |
| Exon14F | CATGTCTGTTTCTCAAAGGG | ||
| Exon14R | CCATAGTCACTGACTGAATTAACAC | 202 | 61 |