Table 1 of Liu, Mol Vis 2011; 17:2129-2138.

Table 1. Sequences of the primers used for reverse transcription polymerase chain reaction.

Primers Sequence (5´→3´) Product size (bp) Annealing temperature (ºC) PCR cycles
ADAMTS-1 (F) CAGTACCAGACCTTGTGCAGACCTT 299 58 37
  (R) CACACCTCACTGCTTACTGGTTTGA      
SDF-1α (F) TGCCCCTGCCGGTTCTTCGAG 311 58 37
  (R) CTGTTGTTGTTCTTCAGCCGTGCAA      
VEGF (F) CTGCTGTACCTCCACCATGCCAAGT 509 57 37
  (R) CTGCAAGTACGTTCGTTTAACTCA      
TGF-β (F) CGGGGCGACCTGGGCACCATCCATGAC 405 57 37
  (R) CTGCTCCACCTTGGGCTTGCGACCCAC      
MMP-2 (F) GAGTTGGCAGTGCAATACCT 666 57 38
  (R) GCCATCCTTCTCAAAGTTGT      
MMP-9 (F) AGTTTGGTGTCGCGGAGCAC 754 57 37
  (R) TACATGAGCGCTTCCGGCAC      
TNF-α (F) CAGCCTCTTCTCATTCCTGCTTGTG 511 58 36
  (R) CTGGAAGACTCCTCCCAGGTATAT      
TSP-1 (F) ACCAAAGCCTGCAAGAAAGA 311 57 37
  (R) ATGCCATTTCCACTGTAGCC      
TSP-2 (F) CAGAGTACTGGCGTCGGTCA 649 57 37
  (R) ATAAGATCGCAGCCCACATACAG      
CXCR4 (F) ATGTAGACACTGGCGGAAATGG 459 57 37
  (R) AGGTGGGGCGAAAGGAAAC      
Actb (F) TGTGATGGTGGGAATGGGTCAG 514 55 25
  (R) TTTGATGTCACGCACGATTTCC      

All primers used were purchased from Genset Oligos (Kyoto, Japan). The amplification was performed using a GeneAmp® PCR System 9700 (Perkin-Elmer, Foster City, CA). In the table, "F" indicates forward primer and "R" indicates reverse primer.

Liu, Mol Vis 2011; 17:2129-2138. http://www.molvis.org/molvis/v17/a231

©2011 Molecular Vision http://www.molvis.org/molvis/

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