Figure 3. c-kit-, F4/80- or Ly-6G-positive cell numbers in cornea after alkali injury. Corneal tissues were obtained 4 days after injury from vehicle- or SDF-1α-treated BABL/c mice, and the tissues from 7 to 8 mice were combined and were subjected to analysis using a flow cytometer after being immunostained with anti-c-kit, anti-F4/80, or anti-Ly-6G antibody. Isotype IgG derived from the same species of the test antibody was used as negative control. Representative results from three to four tests of intracorneal infitlration of c-kit- (A), F4/80- (B), or Ly-6G-positive cells (C) from either vehicle- (left plot) or SDF-1α-treated mice (right plot) are shown.