Table 2 of
Mackey, Mol Vis 2011; 17:2118-2128. Table 2 of
Mackey, Mol Vis 2011; 17:2118-2128.
Table 2. TSPAN12 primers and PCR conditions.
| Exon | Primer names and sequences (5’-3’) | Size (bp) | Annealing temperature | Amplification conditions |
|---|---|---|---|---|
| 2 | TSPAN12-ex2-F ATGTCCCGTGTTCTCTCTCC | 382 | 60 °C | Invitrogen Taq & buffer |
| TSPAN12-ex2-R CCAGGGGTGGATTTCTTTGT | ||||
| 3 | TSPAN12-ex3-F TGGTAATTGGGAAAGATATTATGTAAC | 291 | 60 °C | Invitrogen Taq & buffer |
| TSPAN12-ex3-R CCAAAAGATCAAGGAAGAGCA | ||||
| 4 | TSPAN12-ex4-F TGAGGCATCATGATTGAAAGAA | 346 | 60 °C | Invitrogen Taq & buffer |
| TSPAN12-ex4-R GCTATCACTGCTCCCTAATCTTGT | ||||
| 5 | TSPAN12-ex5-F GGTCCCCTTTCTTGGAGAAC | 947 | 60 °C | Invitrogen Taq & buffer |
| TSPAN12-ex5-R TGGAAATGTGCTTTAGACACAGA | ||||
| 6 | TSPAN12-ex6-F GTACAAAATACCTCTTCATTTATCACA | 529 | 60 °C | Hot shot master mix |
| TSPAN12-ex6-R GAAGAAAAGCAGGCCATGAA | ||||
| 7 | TSPAN12-ex7-F TGATGACAGATATAGCTCTGGGT | 376 | 60 °C | Hot shot master mix |
| TSPAN12-ex7-R TTTTAAGGCCTTTTACATTTAGACA | ||||
| 8 | TSPAN12-ex8-F GCTTTCCCTGAGAACCACTG | 605 | 60 °C | Hot shot master mix |
| TSPAN12-ex8-R CCATCCTCATTTTAAAGCATAGA |