Table 2 of Huang, Mol Vis 2011; 17:186-198.

Table 2. Summary of phosphorylated sites from normal and cataractous lenses.

Protein [accession number] Novel site Designation Fragment Peptide* m/z Charge Score P/N ratio¶
Crystallin proteins
Beta-crystallin B1 [P53674] Yes Ser-32 25–50 GAPPAGTSPSPGTTLAPTTVPITSAK 1229.136 2 94.99 0.32
  Yes Ser-81 73–86 RAEFSGECSNLADR 817.847 2 89.04 0.40
        RAEFSGECSNLADR 545.564 3 69.03 0.46
  Yes Ser-93 91–110 VRSIIVSAGPWVAFEQSNFR 781.732 3 62.53 5.52
Beta-crystallin B2 [P43320]   Thr-91 90–101 RTDSLSSLRPIK 484.894 3 44.22 1.66
  Yes Ser-148 146–160 VQSGTWVGYQYPGYR 920.923 2 110.26 2.69
  Yes Ser-174 169–188 GDYKDSSDFGAPHPQVQSVR 757.332 3 64.55 2.49
  Yes Ser-175 169–188 GDYKDSSDFGAPHPQVQSVR 757.345 3 66.7 2.30
Alpha-crystallin B [P02511] No Ser-19 12–22 RPFFPFHSPSR 485.557 3 68.97 1.89
  No Ser-76 73–82 DRFSVNLDVK 636.793 2 66.09 2.09
Gamma-crystallin D [P07320] Yes Ser-21 16–32 HYECSSDHPNLQPYLSR 709.31 3 49.79 0.29
  Yes Ser-73 61–77 GDYADHQQWMGLSDSVR 1022.934 2 57.89 0.23
  Yes Ser-75 60–77 RGDYADHQQWMGLSDSVR 734.304 3 64.34 0.47
      61–77 GDYADHQQWMGLSDSVR 1022.929 2 79.19 0.21
Alpha-crystallin A [P02489] Yes Thr-153 146–157 IQTGLDATHAER 696.33 2 70.14 4.26
Beta-crystallin S [P22914] Yes Thr-6 4–14 TGTKITFYEDK 691.825 2 46.45 0.44
  Yes Tyr-11 8–19 ITFYEDKNFQGR 799.348 2 74.57 0.48
Gamma-crystallin B [P07316] Yes Ser-75 60–77 RGEYPDYQQWMGLSDSIR 1141.009 2 74.11 0.11
Beta-crystallin A3 [P05813] Yes Ser-209 197–212 EWGSHAQTSQIQSIRR 655.3 3 58.65 5.41
Other proteins
Phosphoglycerate kinase 1 [P00558] Yes Ser-175 172–184 AHSSMVGVNLPQK 732.304 2 48.65 10.70
Uncharacterized protein C3orf72 [Q6ZUU3] Yes Ser-35 32–40 LSESPALVK 512.237 2 39.15 4.84
Quinone oxidoreductase PIG3 [Q53FA7] No Ser-260 258–267 RGSLITSLLR 598.324 2 50.37 4.29
Aquaporin-5 [P55064] Yes Thr-397 395–403 KKTMELTTR 594.28 2 47.93 3.48
Peroxiredoxin-2 [P32119] No Ser-112 110–119 RLSEDYGVLK 630.302 2 40.74 3.18
Malate dehydrogenase [P40925] No Ser-241 239–248 KLSSAMSAAK 545.263 2 43.74 2.46
Pyruvate kinase isozymes M1/M2 [P14618] Yes Ser-249 247–255 KASDVHEVR 560.771 2 51.31 2.12
Phakinin [Q13515] Yes Ser-34 32–43 SSSSLESPPASR 642.748 2 52.18 0.46
Filensin [Q12934] Yes Ser-454 452–467 VRSPKEPETPTELYTK 652.331 3 64.65 0.35
Glyceraldehyde-3-phosphate dehydrogenase [P04406] No Thr-184 163–186 VIHDNFGIVEGLMTTVHAITATQK 897.788 3 58.72 0.29
Heat shock protein beta-1 [P04792] No Ser-82 80–89 QLSSGVSEIR 578.283 2 43.24 0.15

The lens proteins analyzed herein were from normal (30-year-old) and cataractous (68-year-old, Grade III of nuclear sclerosis) human lenses obtained post mortem from Department of Ophthalmology, Chang Gung Memorial Hospital (J.-S. Lee). This table shows the ratio of identified phosphorylated peptides in cataractous and normal human lenses by quantitative phosphoproteomic analysis. *Phosphorylation sites are underlined. ¶P, cataract patients; N, normal without cataract.

Huang, Mol Vis 2011; 17:186-198. http://www.molvis.org/molvis/v17/a23

©2011 Molecular Vision http://www.molvis.org/molvis/

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