Molecular Vision: Huang, Mol Vis 2011; 17:186-198. Table 1

Table 1. Summary of identified phosphorylated proteins and phosphorylated sites in human lens proteins.

Protein [Accession number]	Protein Mass, kDa	Fragment	Phosphopeptides*	Designation
Crystallin proteins
Alpha-crystallin B [P02511]	20.146	12–22	RPFFPFHSPSR	Ser-19
		12–22	RPFFPFHSPSR	Ser-21
		57–69	APSWFDTGLSEMR	Ser-59
		73–82	DRFSVNLDVK	Ser-76
		124–149	IPADVDPLTITSSLSSDGVLTVNGPR	Ser-139
Alpha-crystallin A [P02489]	19.897	13–21	TLGPFYPSR	Thr-13
		55–70	TVLDSGISEVRSDRDK	Ser-66
		79–88	HFSPEDLTVK	Ser-81
		146–157	IQTGLDATHAER	Thr-148
		146–157	IQTGLDATHAER	Thr-153
Beta-crystallin B1 [P53674]	28.006	25–50	GAPPAGTSPSPGTTLAPTTVPITSAK	Ser-32
		73–86	RAEFSGECSNLADR	Ser-77
		73–86	RAEFSGECSNLADR	Ser-81
		91–110	VRSIIVSAGPWVAFEQSNFR	Ser-93
		93–110	SIIVSAGPWVAFEQSNFR	Ser-97
		93–110	SIIVSAGPWVAFEQSNFR	Ser-107
		188–202	VSSGTWVGYQYPGYR	Ser-189
		188–202	VSSGTWVGYQYPGYR	Ser-190
		203–214	GYQYLLEPGDFR	Tyr-204
Beta-crystallin B2 [P43320]	23.365	90–101	RTDSLSSLRPIK	Thr-91
		90–101	RTDSLSSLRPIK	Ser-93
		91–101	TDSLSSLRPIK	Ser-95
		109–120	IILYENPNFTGK	Tyr-112
		109–120	IILYENPNFTGK	Thr-118
		146–160	VQSGTWVGYQYPGYR	Ser-148
		169–188	GDYKDSSDFGAPHPQVQSVR	Ser-174
		169–188	GDYKDSSDFGAPHPQVQSVR	Ser-175
Beta-crystallin A3 [P05813]	25.134	46–64	MEFTSSCPNVSERSFDNVR	Thr-49
		197–211	EWGSHAQTSQIQSIR	Ser-200
		197–212	EWGSHAQTSQIQSIRR	Ser-209
Beta-crystallin A4 [P53673]	22.360	49–71	VLSGAWVGFEHAGFQGQQYILER	Ser-51
		104–118	DSRLTIFEQENFLGK	Thr-108
Beta-crystallin S [P22914]	20.993	4–14	TGTKITFYEDK	Thr-6
		8–19	ITFYEDKNFQGR	Tyr-11
		85–95	AVHLPSGGQYK	Ser-90
		159–174	KPIDWGAASPAVQSFR	Ser-167
Gamma-crystallin B [P07316]	20.894	60–77	RGEYPDYQQWMGLSDSIR	Ser-75
Gamma-crystallin D [P07320]	20.725	16–32	HYECSSDHPNLQPYLSR	Ser-21
		61–77	GDYADHQQWMGLSDSVR	Ser-73
		61–77	GDYADHQQWMGLSDSVR	Ser-75
		153–163	RYQDWGATNAR	Thr-160
Other proteins
Filensin [Q12934]	74.499	5–11	SYVFQTR	Ser-5
		230–239	EVLSHLQAQR	Ser-233
		452–467	VRSPKEPETPTELYTK	Ser-454
		454–467	SPKEPETPTELYTK	Thr-462
		457–467	EPETPTELYTK	Thr-460
		605–615	SRSLPEKGPPK	Ser-605
		607–615	SLPEKGPPK	Ser-607
Phakinin [Q13515]	45.851	32–43	SSSSLESPPASR	Ser-34
		77–89	ALGISSVFLQGLR	Ser-81
		77–89	ALGISSVFLQGLR	Ser-82
Fructose-bisphosphate aldolase C [P09972]	48.378	116–129	GILAADESVGSMAK	Ser-26
		130–144	RLSQIGVENTEENRR	Ser-132
Phosphoglycerate kinase 1 [P00558]	44.586	172–184	AHSSMVGVNLPQK	Ser-175
Heat shock protein beta-1 [P04792]	22.768	80–89	QLSSGVSEIR	Ser-82
Quinone oxidoreductase PIG3 [Q53FA7]	35.514	258–267	RGSLITSLLR	Ser-260
Aquaporin-5 [P55064]	41.943	395–403	KKTMELTTR	Thr-397
Peroxiredoxin-2 [P32119]	21.878	110–119	RLSEDYGVLK	Ser-112
Malate dehydrogenase [P40925]	19.897	239–248	KLSSAMSAAK	Ser-241
Pyruvate kinase isozymes M1/M2 [P14618]	58.025	247–255	KASDVHEVR	Ser-249
Coronin-1B [Q9BR76]	54.200	207–214	RGTLVAER	Thr-209
Actin-related protein 2/3 complex subunit 2 [O15144]	22.724	135–148	RASHTAPQVLFSHR	Thr-139
Plectin-1 [Q15149]	531.466	3783–3793	RLTAEDLFEAR	Thr-3785
Limbic system-associated membrane protein [Q13449]	37.370	89–97	RHSLEYSLR	Ser-91
Drebrin [Q16643]	71.385	140–147	LSSPVLHR	Ser-142
Ras-related C3 botulinum toxin substrate 2 [P15153]	21.415	164–174	GLKTVFDEAIR	Thr-167
Band 4.1-like protein 2 [O43491]	112.519	400–412	RLSMYGVDLHHAK	Ser-402
Nucleoside diphosphate kinase A [P15531]	17.138	89–105	VMLGETNPADSKPGTIR	Thr-94
Retinal dehydrogenase 1 [P00352]	54.827	411–419	FKSLDDVIK	Ser-413
Fructose-bisphosphate aldolase A [P04075]	39.395	29–43	GILAADESTGSIAKR	Ser-39
Glyceraldehyde-3-phosphate dehydrogenase [P04406]	36.030	163–186	VIHDNFGIVEGLMTTVHAITATQK	Thr-184
Lens fiber major intrinsic protein [P30301]	28.104	229–238	SISERLSVLK	Ser-235
Uncharacterized protein C3orf72 [Q6ZUU3]	18.613	32–40	LSESPALVK	Ser-35