Figure 1 of Huang, Mol Vis 2011; 17:186-198.


Figure 1. Comparative analysis of normal and cataractous human lens proteins by SDS–PAGE followed by LC-nanoESI-MS/MS. As shown in the left panel, a total of 10 μg lens proteins derived from normal (N) and cataractous (P) eye lenses were resolved with 12.5% SDS–PAGE and stained with Coomassie brilliant blue R-250. In the right panel, protein and peptide bands with different expression levels identified by LC-nanoESI-MS/MS were indicated by arrows. In comparison with normal human lens proteome, four crystallin proteins, β-crystallin B1, β-crystallin B2, βs-crystallin, and γD-crystallin in the cataract lens were found to significantly decrease in expression levels as compared to normal lens.