Figure 7. LY294002 did not affect the upregulated HSP70 expression induced by sodium selenite. The intervention duration of LY294002, sodium selenite and 1,2-DHN was 1, 12, and 24 h, respectively. The expression of HSP70 at the mRNA and protein levels was quantified by RT–PCR and western blot analysis, respectively. Each experiment was performed in triplicate.