Figure 5. Sodium selenite inhibited
1,2-DHN-induced apoptosis in hLECs through activation of PI3-K/Akt
pathway. Apoptosis was evaluated using Annexin V/PI staining and flow
cytometry assay. Each experiment was performed in triplicate. A:
Control
group. Early apoptosis: 4.43±0.31%. B: Cultured hLECs
were treated with 5×10−5 M 1,2-DHN for 24 h. Early
apoptosis: 25.4±0.95%. C: Cultured hLECs were incubated with 4
ng/ml sodium selenite for 12 h before the 24-h treatment of 5×10−5
M 1,2-DHN. Early apoptosis: 11.92±0.86%. D: Cultured hLECs were
first treated with 30 μM LY294002 for 1 h, and then the cells were
incubated with 4 ng/ml sodium selenite for 12 h before the 24-h
treatment of 5×10−5 M 1,2-DHN. Early apoptosis: 28.12±0.96%.