Figure 5 of Zhu, Mol Vis 2011; 17:2019-2027.


Figure 5. Sodium selenite inhibited 1,2-DHN-induced apoptosis in hLECs through activation of PI3-K/Akt pathway. Apoptosis was evaluated using Annexin V/PI staining and flow cytometry assay. Each experiment was performed in triplicate. A: Control group. Early apoptosis: 4.43±0.31%. B: Cultured hLECs were treated with 5×10−5 M 1,2-DHN for 24 h. Early apoptosis: 25.4±0.95%. C: Cultured hLECs were incubated with 4 ng/ml sodium selenite for 12 h before the 24-h treatment of 5×10−5 M 1,2-DHN. Early apoptosis: 11.92±0.86%. D: Cultured hLECs were first treated with 30 μM LY294002 for 1 h, and then the cells were incubated with 4 ng/ml sodium selenite for 12 h before the 24-h treatment of 5×10−5 M 1,2-DHN. Early apoptosis: 28.12±0.96%.