Figure 4. Immunofluorescence staining results also showed that sodium selenite induced Akt phosphorylation in a PI3-K-dependent manner either in the normal or 1,2-DHN stimulated state. The intervention duration of LY294002, sodium selenite and 1,2- DHN was 1, 12, and 24 h, respectively. It was noteworthy that pretreatment of 4 ng/ml sodium selenite apparently promoted Akt phosphorylation and nuclear translocation in hLECs treated with 5×10⁻⁵ M 1, 2-DHN. This effect was inhibited by 1-h pretreatment of 30 μM LY294002.