Figure 1. Sodium selenite increased cell viability and protected hLECs from 1,2-DHN-induced cell death. A: Cultured hLECs were treated with 1,2-DHN at different concentrations for 24 h. Cell viabilities were assayed using the CCK-8 kit. B: Cultured hLECs were either treated with sodium selenite alone at different concentrations for 12 h or given a combined treatment with 5×10⁻⁵ M 1,2-DHN for 24 h. C: Cultured hLECs were treated with 1,2-DHN alone at different concentrations for 24 h or given a pretreatment with 4 ng/ml sodium selenite for 12 h before the 1,2-DHN treatment. The relative survival rate was estimated by tryphan blue exclusion assay. D: Inverted microscope images of hLECs treated with 1,2-DHN at two concentrations with or without 4 ng/ml sodium selenite pretreatment. (*p<0.05, **p<0.01) Each experiment was performed in triplicate.