Figure 2. TGF-β induces expression of SPARC in human Tenon’s fibroblasts in vitro. Representative northern blot analysis (A, B) and densitometry (C) for SPARC expression in HTF cells without and with incubation with activated TGF-β1 (1 ng/ml) or TGF-β2 (1 ng/ml) for three days. For quantification, SPARC mRNA levels were measured densitometrically, normalized to 28S methylene blue staining and expressed as ×-fold to levels of untreated control cells (mean±SEM of 3 independent experiments; *p<0.05). Representative western blot analysis (D, E) and densitometry (F) in HTF cells for SPARC without and with incubation with activated TGF-β1 (1 ng/ml) or TGF-β2 (1 ng/ml) for three days. For quantification, SPARC protein levels were measured densitometrically, normalized to coomassie blue staining and expressed as ×-fold to levels of untreated control cells (mean±SEM of 3 independent experiments; *p<0.05).