Figure 4. Zbed4 does not co-localize with green and blue opsins in adult mouse and rat retinas. Confocal microscopy of mouse (A) and rat (B) retinal sections were double labeled with antibodies against Zbed4 and green/red or blue opsin, followed by Alexa 488 goat anti-rabbit IgG (green) and Alexa 568 goat anti-chicken IgG (red). The sections were then stained with DAPI (blue). Cone outer segments are stained red by opsin antibodies. Magnification is 400×. Acronyms: OS, outer segments; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer.