Figure 4. Antioxidative effects of 17β-estradiol against H₂O₂ exposure. Human lens epithelial cells (HLECs) were preincubated with 17β-estradiol for 4 h and then simultaneously exposed to 25 µM H₂O₂ and 17β-estradiol for 24 h. A: A decrease in peroxide levels at low concentrations of 17β-estradiol (0.01–1 µM) was demonstrated. B: Superoxide levels were decreased at 0.01–1 µM 17β-estradiol. Mean±SEM is shown. Experiments were performed three times in triplicates (n=3) and one representative experimental run for each method is shown. *p<0.05 as compared to control without 17β-estradiol (0) exposure.