Table 1 of Mamatha, Mol Vis 2011; 17:1970-1977. Table 1 of Mamatha, Mol Vis 2011; 17:1970-1977.
Table 1. Coding exons and flanking intronic regions of CYP4V2 and exon 5 of TIMP3 primers and conditions.
Exon number |
Primer sequence (5′-3′) |
Annealing temperature (°C) |
|---|---|---|
| Exon 1 | F: CAACCTCGCAGCACCCTCAGAA | 62 |
| R: ACTTTGGGATGGGGCACTAGCAGT | ||
| Exon 2 | F: ACCTGGCTTCCTCTAACAGTAACA | 60 |
| R: TTTTTGTGCTGAAATGGCTGAA | ||
| Exon 3 | F: AGATTCGCCTCCTCCCACCTCAC | 67 |
| R: ACCTGGACTCTTGGCCTCTTGACG | ||
| Exon 4 | F: TGCCAAAAGCATTTGAGAACCTGT | 63 |
| R: CGCGCTGAAGAGCCCGTCAC | ||
| Exon 5 | FPAGGAAGAACAGGAACAGGGAGTAG | 63 |
| R: CAACGCAGAAATTGTTAGCAATAA | ||
| Exon 6 | F: GCTTCATGGGATGCGTAATAGC | 60 |
| R: GAAATGAACGGTGGGGATGGT | ||
| Exon 7 | F: CCTATGTTGTCGAAATGTTGAAAT | 52* |
| R: TCTGAAGAAGTTGAGCTGGTACTT | ||
| Exon 8 | F: TTGCAGTCACAGTGCAGTCATCA | 67 |
| R: CCAGCATCCGGCCTAGTACAGTC | ||
| Exon 9&10 | F: ATGCCATGCCTTGATCCACCTGT | 63 |
| R: TGGGCAATGTCACATCACATCTCA | ||
| Exon 11 | F: CTCTTCATCTTTAACAGGTGTTCC | 65 |
| R: CAAAACTCAAAACTTTTTCTTTGT |