Figure 5. Growth assay of hSTI1(K434R)/UTP21
double mutants. Combining specific sti1--interacting
mutations of yeast UTP21 with the hSTI1(K434R) variant
decreases the growth rate of liquid cultures at 37 °C. Although
expression of the glaucoma-associated K434R variant does not affect
growth in the WT or L5P UTP21 backgrounds, co-expression with
the mutations UTP21(S602F) or UTP21(D621G)
significantly decreased the growth rate (asterisk, t-Test of linear
slopes, p<0.05, 4 experiments).