Figure 3. Radicicol sensitivity assay. A
null mutation at the yeast STI1 locus confers sensitivity to
the HSP90 inhibitor radicicol (RD) resulting in slow growth at
30 °C on rich media (asterisks). The glaucoma-associated STI1
variant K434R and the yeast-equivalent K470R do not alter growth on RD
plates. Co-expression of yeast UTP21 mutations (L5P, S602F,
D621G) which genetically interact with sti1- does
not affect sensitivity to RD, either alone or in combination with hSTI1(K434R).